Ikaros is required for plasmacytoid dendritic cell differentiation. Commentary

摘要: Plasmacytoid dendritic cells (pDCs) are specialized DCs that produce high levels of type I IFN upon viral infection. Despite their key immunoregulatory role, little is known about pDC ontogeny or how developmental events regulate function. We show mice expressing low the transcription factor Ikaros (Ik L/L ) lack peripheral pDCs, but not other DC subsets. Loss pDCs associated with an inability to after challenge Toll-like receptor-7 and -9 ligands, murine cytomegalovirus (MCMV) In contrast, conventional present in normal numbers exhibit responses vivo MCMV inactivated toxoplasma antigen. Interestingly, Ik bone marrow (BM) contain a population appears blocked at Ly-49Q - stage differentiation fails terminally differentiate response Flt-3L, cytokine required for differentiation. This block strictly dependent on cell-intrinsic requirement pDC-committed precursors. Global gene expression profiling BM reveals up-regulation genes normally expressed, expressed levels, WT pDCs. These studies suggest controls by silencing large array genes.