Glycosaminoglycan synthesis in the rat articular disk in response to mechanical stress.

摘要: The mechanism by which compressive mechanical stress affects glycosaminoglycan synthesis in the articular disk was investigated with a modified organ culture technique. Forty-eight male Sprague-Dawley rats were divided into three experimental groups and one control group of 12 animals each, aged 7 9 weeks. followed different regimens applied for 25%, 75%, or 100% time during total test period 24 hours. Articular disks stressed flexible bottomed dishes (Flex I dishes, Flexcell Corp., McKeesport, Pa.) using Flexercell Strain Unit (Flexcell incubated [3H]-glucosamine Samples then collected, digested Pronase-E, after precipitation cetylpyridinium chloride (CPC) ethanol, glycosaminoglycans (GAGs) separated cellulose acetate electrophoresis. significant GAG types chondroitin6sulfate (C6S), hyaluronic acid (HA), dermatan sulfate (DS). There no relationship between age regimen either age. Higher showed significantly higher proportions C6S when compared controls, whereas HA appeared to decrease slightly DS not affected. Since is major component hyaline cartilage, results this study suggest that forces may stimulate development more cartilagenous-like properties respect content.