High-resolution semi-quantitative real-time PCR without the use of a standard curve.
作者： Alex Gentle , Frank Anastasopoulos , Neville A. McBrien
DOI: 10.2144/01313ST03
关键词:
摘要: … The data analysis is performed in such a fashion that it circumvents the need for a standard curve, thus saving on reagents and allowing more samples to be assayed in each run. …
future-science.com
unirioja.es
sci-hub.se 参考文章(6)
Carl T. Wittwer, Mark G. Herrmann, Alan A. Moss, Randy P. Rasmussen, Continuous fluorescence monitoring of rapid cycle DNA amplification. BioTechniques. ,vol. 22, pp. 130- 138 ,(1997) , 10.2144/000114043
F Ferre, Quantitative or semi-quantitative PCR: reality versus myth. Genome Research. ,vol. 2, pp. 1- 9 ,(1992) , 10.1101/GR.2.1.1
Jarle Skeidsvoll, Per Magne Ueland, Analysis of Double-Stranded DNA by Capillary Electrophoresis with Laser-Induced Fluorescence Detection Using the Monomeric Dye SYBR Green I Analytical Biochemistry. ,vol. 231, pp. 359- 365 ,(1995) , 10.1006/ABIO.1995.9986
Franz Ludwig Dumoulin, Hans Dieter Nischalke, Ludger Leifeld, Annette von dem Bussche, Jürgen Kurt Rockstroh, Tilman Sauerbruch, Ulrich Spengler, Semi-quantification of human C-C chemokine mRNAs with reverse transcription/real-time PCR using multi-specific standards. Journal of Immunological Methods. ,vol. 241, pp. 109- 119 ,(2000) , 10.1016/S0022-1759(00)00210-6
Carl T. Wittwer, Tom B. Morrison, Janis J. Weis, Quantification of low-copy transcripts by continuous SYBR® Green I monitoring during amplification BioTechniques. ,vol. 24, pp. 954- 962 ,(1998)
D. G. Altman, J. M. Bland, Measurement in Medicine: The Analysis of Method Comparison Studies The Statistician. ,vol. 32, pp. 307- 317 ,(1983) , 10.2307/2987937