Recognition of human insulin in the context of HLA-DRB1*0406 products by T cells of insulin autoimmune syndrome patients and healthy donors.

摘要: Our recent study indicated that all the insulin autoimmune syndrome (IAS) patients had specific HLA class II alleles, DRB1*0406, DQA1*0301, and DQB1*0302, which allowed T cells to proliferate when autologous APC were exposed human insulin. The implied gene products of and/or DQB1*0302 may be involved in presentation cells. We therefore examined cell response healthy donors with different phenotypes using an MLR system. from not only IAS but also able after exposure products. molecules are considered recognition by proliferative was completely blocked anti-HLA-DR mAb anti-HLA-DQ or other mAb. Furthermore, insulin-specific CD4-positive clones established blast PBMC two DRB1*0406 presence Using DRB1*0406-transfected L as APC, we confirmed these recognize context DRB1*0406. These results provide first evidence HLA-DRB1*0406 act dominant restriction element for cells, suggest this particular gene, HLA-DRB1*0406, contributes development IAS.