Physical Entrapment of Adriamycin in AB Block Copolymer Micelles
作者： Glen S. Kwon , Mayumi Naito , Masayuki Yokoyama , Teruo Okano , Yasuhisa Sakurai
关键词:
摘要: The entrapment of Adriamycin (ADR) in micelles composed AB block copolymers (poly(ethylene oxide-co-β-benzyl L-aspartate) (PEO-PBLA)) was investigated. loading process involved transfer ADR and PEO-PBLA into an aqueous milieu from dimethyl-formamide (DMF) through a dialysis procedure. Evidence for the physical polymeric derived fluorescence spectroscopy gel permeation chromatography (GPC). total intensity low, suggesting that drug self-associated micelles. In addition, quenching experiments, using water-soluble quencher (iodide (I–)), showed present micellar solutions largely unaffected by I–, whereas free readily quenched. From Stern-Volmer plots, constants (KSV) 2.2 17 M−l were determined ADR, respectively. As result micelles, binds only slightly serum albumin as evidenced GPC. contrast, solutions. findings suggest is stably entrapped expected to affect markedly pharmacokinetics ADR.
springer.com
sci-hub.se 参考文章(14)
Glen S. Kwon, Masayuki Yokoyama, Teruo Okano, Yasuhisa Sakurai, Kazunori Kataoka, Biodistribution of micelle-forming polymer-drug conjugates Pharmaceutical Research. ,vol. 10, pp. 970- 974 ,(1993) , 10.1023/A:1018998203127
Alberto A. Gabizon, Yechezkel Barenholz, Meir Bialer, Prolongation of the circulation time of doxorubicin encapsulated in liposomes containing a polyethylene glycol-derivatized phospholipid: pharmacokinetic studies in rodents and dogs. Pharmaceutical Research. ,vol. 10, pp. 703- 708 ,(1993) , 10.1023/A:1018907715905
Glen S. Kwon, Mayumi Naito, Kazunori Kataoka, Masayuki Yokoyama, Yasuhisa Sakurai, Teruo Okano, Block copolymer micelles as vehicles for hydrophobic drugs Colloids and Surfaces B: Biointerfaces. ,vol. 2, pp. 429- 434 ,(1994) , 10.1016/0927-7765(94)80007-3
Sherwin Lehrer, Solute perturbation of protein fluorescence. The quenching of the tryptophyl fluorescence of model compounds and of lysozyme by iodide ion. Biochemistry. ,vol. 10, pp. 3254- 3263 ,(1971) , 10.1021/BI00793A015
Gregory S. Karczmar, Thomas R. Tritton, The interaction of adriamycin with small unilamellar vesicle liposomes. A fluorescence study Biochimica et Biophysica Acta. ,vol. 557, pp. 306- 319 ,(1979) , 10.1016/0005-2736(79)90329-8
Masayuki Yokoyama, Glenn S. Kwon, Teruo Okano, Yasuhisa Sakurai, Takashi Seto, Kazunori Kataoka, Preparation of micelle-forming polymer-drug conjugates. Bioconjugate Chemistry. ,vol. 3, pp. 295- 301 ,(1992) , 10.1021/BC00016A007
Kazunori Kataoka, Glenn S Kwon, Masayuki Yokoyama, Teruo Okano, Yasuhisa Sakurai, Block copolymer micelles as vehicles for drug delivery Journal of Controlled Release. ,vol. 24, pp. 119- 132 ,(1993) , 10.1016/0168-3659(93)90172-2
Dan W. Urry, Shao Q. Peng, Timothy M. Parker, D. Channe Gowda, Roland D. Harris, Relative Significance of Electrostatic- and Hydrophobic-Induced pKa Shifts in a Model Protein: The Aspartic Acid Residue Angewandte Chemie. ,vol. 32, pp. 1440- 1442 ,(1993) , 10.1002/ANIE.199314401
Martin Malmsten, Bjoern Lindman, Self-assembly in aqueous block copolymer solutions Macromolecules. ,vol. 25, pp. 5440- 5445 ,(1992) , 10.1021/MA00046A049
Rachel Goldman, Terenzio Facchinetti, Diana Bach, Avraham Raz, Meir Shinitzky, A differential interaction of daunomycin, adriamycin and their derivatives with human erythrocytes and phospholipid bilayers Biochimica et Biophysica Acta. ,vol. 512, pp. 254- 269 ,(1978) , 10.1016/0005-2736(78)90251-1