Identification of a very early promoter of insect Hz-1 virus using a novel dual-expression shuttle vector

摘要: Very early promoters of viruses control the proper cascade expression viral genes and are essential for completion virus life cycles. These usually rare weak do not encode structural proteins. As a result, they difficult to identify. In order identify clone very large eukaryotic DNA virus, Hz-1 novel cloning strategy was applied. This is based on dual-expression shuttle vector containing promoter-less lacZ gene. Insertion upstream permits efficient LacZ in bacteria cells. The function putative then confirmed by their insect first two productive infection-specific contained within vectors pTSV-2-129 pTSV-2-49, were cloned from HindIII-K HindIII-A fragments genome, respectively. By primer extension analysis, an immediate constitutive promoter detected after infection. Identification has laid down important groundwork future studies molecular mechanism transcriptional switch between persistent infections virus.