Mass Spectrometric Analysis of Surfactant Metabolism in Human Volunteers Using Deuteriated Choline
作者： Anthony D. Postle , Wolfgang Bernhard , Christopher J. Pynn , Andreas Jaworski , Gunnar A. Rau
DOI: 10.1164/RCCM.200401-089OC
关键词:
摘要: Surfactant reduces surface tension at pulmonary air–liquid interfaces. Although its major component is dipalmitoyl–phosphatidylcholine (PC16:0/16:0), other PC species, principally palmitoylmyristoyl–PC, palmitoylpalmitoleoyl–PC, and palmitoyloleoyl–PC, are integral components of surfactant. The composition metabolism species depend on development, respiratory rate, pathologic alterations, which have largely been investigated in animals using radiolabeled precursors. Recent advances mass spectrometry availability precursors carrying stable isotopes make metabolic experiments human subjects ethically feasible. We introduce a technique to quantify surfactant synthesis vivo deuteriated choline coupled with electrospray ionization tandem spectrometry. Endogenous from induced sputa healthy volunteers comprised 54.0 ± 1.5% PC16:0/16:0, 9.7 0.7% 10.0 1.0% 13.1 0.3% palmitoyloleoyl–PC. Infusion of...
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