High Order Autocorrelation in Fluorescence Correlation Spectroscopy

摘要: Fluorescence correlation spectroscopy (FCS) was originally developed as a technique in which the temporal fluctuations fluorescence arising from small volume containing fluorescent molecules are autocorrelated to obtain information about processes that give rise fluctuations. After its initial introduction [21.1-21.6], FCS used primarily measure translational diffusion coefficients [21.7-21.10]. It also shown could provide other such rotational [21.11-21.14], flow [21.15], and biochemical kinetics [21.16,21.17]. These applications were comprehensively reviewed number of years ago [21.18- 21.21]. has become increasingly mature during last decade, both terms application development. Recent include characterization receptor oligomerization on intact cells [21.22]; aggregates prion proteins [21.23] amyloid /3-proteins [21.24]; DNA polymerization [21.25], conformational [21.26], interaction with transcription factors [21.27]; phospholipid micelles [21.28]. Some more innovative technological advances have included use two-photon excitation [21.29,21.30]; cross-correlation double-label experiments [21.31- 21.35]; combination resonance energy transfer [21.26,21.36]; capillary electrophoresis [21.37]; evanescent illumination for examining at surfaces [21.38-21.42].