Morphogenesis and plantlet regeneration from hypocotyl explants in three selected species of Citrus.

摘要: Hypocotyls of three species Citrus were cultured on different fortifications MS media to assess their in vitro response. Higher in vitro morphogenesis (somatic embryogenesis and/or embryogenesis) leading plantlet regeneration was varied considerably due and inoculation medium. Induction medium MS4D.5B (MS+ 4.0 mg/l 2, 4-D + 0. 5 BAP) proved well for callus initiation. Nutrient MS4B.5N (MS mg/l, BAP 0.5 mg.l-1 NAA) performed commandingly formation morphogenic calli regeneration. Higher root proliferating efficiency recorded rooting MS.5I mg. l-1 IBA). Roots higher numbers attained from culture MS2I.5Kn 2. 0 IBA Kn), while MS.5I.5B supported enhancing mean length. In terms inter specific in vitro response, general, Acid lime followed by Mandarin Sweet orange responded decisively the most phases. The raised plantlets acclimatized established successfully field.