In vitro measurement of available iron in fortified foods
作者： R Wolfgor , S.R Drago , V Rodriguez , N.R Pellegrino , M.E Valencia
DOI: 10.1016/S0963-9969(01)00122-3
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摘要: Abstract The objective of this study was to investigate the influence base systems used regulate pH values digests on iron dialysability as an indicator bioavailable iron. We studied pertinence measuring titratable acidity 7.5 with KOH calculate mEq NaHCO 3 be for pancreatic digestion/dialysis. achieved using same each lower when than KOH. discrepancy between and attempted uneven several sources. Differences in regulation procedure, including type concentration or buffer added pepsin digest rendered different final digest/dialysate values, thus affecting dialysable A modification vitro equilibrium dialysis method is proposed PIPES sufficient molarity obtain a uniform 6.5 systems. main factors taken into account were capacity food matrix (HCl required reach 2), HCl included aliquot suspension, acid generated through enzymatic hydrolysis during digestion intrinsic adjust 6.5). With these data can calculated. Modifications suggested allowed development system variety foods assayed.
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