Evidence of TRPV1 receptor and PKC signaling pathway in the antinociceptive effect of amyrin octanoate.

摘要: Previous studies from our group investigated the antinociceptive property of amyrin octanoate, a synthetic compound derivative natural precursor alpha, beta-amyrin, against nociceptive response induced by acetic acid and formalin. Here, we some mechanisms action underlying effects octanoate. Amyrin octanoate given intraperitoneally (0.001-1 mg /kg) or intrathecally (10-1000 ng /site) caused dose-dependent long-lasting inhibition acid-induced visceral nociception, with mean ID(50) values 0.003 (0.001-0.005) mg/kg 122.4 (60.8-246.6) ng/site, respectively. In capsaicin- glutamate-induced paw licking, significant both responses, 1.36 0.04 mg/kg, Furthermore, also reduced significantly nociception intrathecal injection glutamate, substance P capsaicin, inhibitions 36+/-11%, 67+/-10% 78+/-5%, The antinociception in test was attenuated neonatal pretreatment mice but seems to involve independent G(i/o) protein, opioidergic, serotonergic, noradrenergic cholinergic system, since it not affected pertussis toxin, naloxone, yohimbine, mecamylamine atropine. addition, thermal mechanical hyperalgesia bradykinin phorbol myristate acetate rats, without affecting similar responses prostaglandin E(2). Taken together, present results shown that produces antihyperalgesic effects, through an interaction capsaicin-sensitive fibers PKC signaling pathway.