Gene for spiralin, the major membrane protein of the helical mollicute Spiroplasma citri: cloning and expression in Escherichia coli.

摘要: A library of cloned Spiroplasma citri genomic sequences was constructed by incorporating HindIII digestion fragments into the plasmid vector pBR328. Immunological screening allowed identification a recombinant containing gene for spiralin, major membrane protein S. citri. The spiralin produced Escherichia coli transformant characterized immunological detection with monoclonal antibody after Western blotting two-dimensional (isoelectric focusing and sodium dodecyl sulfate-polyacrylamide) electrophoresis gels partial proteolytic mapping. occurred within 6.5-kilobase-pair DNA fragment. Spiralin in E. regardless orientation insert pBR328 vector. spiroplasmal sequence which acted as promoter along structural is expressed from that sequence.