Abrogation of nontypeable Haemophilus influenzae Protein D function reduces phosphorylcholine decoration, adherence to airway epithelial cells, and fitness in a chinchilla model of otitis media
作者： Ryan W. Johnson , Glen McGillivary , Philippe Denoël , Jan Poolman , Lauren O. Bakaletz
DOI: 10.1016/J.VACCINE.2010.12.003
关键词:
摘要: The pneumococcal polysaccharide conjugate vaccine which includes a nonacylated protein D carrier from Haemophilus influenzae has been recently licensed for use in many countries. While this is protective against nontypeable (NTHI)-induced acute otitis media (OM), the mechanism underlying efficacy not yet fully understood. Protein D/glycerophosphodiester phosphodiesterase (PD/GlpQ) an outer membrane lipoprotein expressed by NTHI that ascribed several functions, including host cell adherence and phosphorylcholine (PCho) acquisition. We found pd/glpQ mutant exhibited reduced to airway epithelial cells, diminished decoration of biofilms, compromised fitness during experimental OM compared parent strain. also exposure antibodies directed formulation recapitulated PCho phenotypes PD/GlpQ-deficient NTHI, providing at least two likely mechanisms polysaccharide-PD/GlpQ induces protection NTHI-induced OM.
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