Identification and Characterization of Botrytis Blossom Blight of Japanese Plums Caused by Botrytis cinerea and B. prunorum sp. nov. in Chile

摘要: Blossom blight is a destructive disease of plums (Prunus salicina) when humid and temperate weather conditions occur in Chile. Disease incidence ranging from 4 to 53% has been observed. Symptoms include light brown petal necrosis, starting as mottles or V-shaped necrosis at the margins petals, progressing stamen pistils. In this study, etiology blossom was determined. High- low-sporulating isolates Botrytis were obtained consistently blighted blossoms apparently healthy flowers plums. Based on colony morphology, conidial production molecular phylogenetic analysis, these high- identified B. cinerea prunorum sp. nov., respectively. Phylogenetic analysis genes glyceraldehyde 3-phosphate dehydrogenase (G3PDH), heat-shock protein 60 (HSP60), DNA-dependent RNA polymerase subunit II (RPB2) grouped single cluster, distantly other species. The ethylene-inducing (NEP1 NEP2) corroborated results. Analysis internal transcribed spacer region large-subunit (26S) ribosomal DNA detection Boty Flipper transposable elements, not useful differentiate between Both species pathogenic plum fruit plums, apples, kiwifruits. However, less virulent than cinerea. These pathogens re-isolated inoculated diseased tissues; thus, Koch's postulates fulfilled, confirming its role predominant, suggesting that may play secondary epidemiology This study clearly demonstrated caused by prunorum, which constitute complex living sympatry possibly stone trees.