A New Method and Mass-Spectrometric Instrument for Extraterrestrial Microbial Life Detection Using the Elemental Composition Analyses of Martian Regolith and Permafrost/Ice.
作者： G.G. Managadze , A.A. Safronova , K.A. Luchnikov , E.A. Vorobyova , N.S. Duxbury
关键词:
摘要: We propose a new technique for the detection of microorganisms by elemental composition analyses sample extracted from regolith, permafrost, and ice extraterrestrial bodies. also describe design ABIMAS instrument, which consists onboard time-of-flight laser mass-reflectron (TOF LMR) preparation unit (SPU) biomass extraction. This instrument was initially approved to fly on board ExoMars 2020 lander mission. The can be used analyze possible microbial communities compare it that terrestrial microorganisms. have conducted numerous laboratory studies confirm possibility identification via following biomarkers: P/S Ca/K ratios, C N abundances. underline only combination these factors will allow one discriminate samples geological ones. Our has been tested experimentally in trials cultures polar permafrost as analogues martian soils. discuss various methods extracting preparation. developed search identify different subsurface other planets, satellites, comets, asteroids-in particular, Europa, Ganymede, Enceladus. Key Words: Mass spectrometry-Life-detection instruments-Biomarkers-Earth Mars-Biomass spectra. Astrobiology 17, 448-458.
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A. L. Mulyukin, V. V. Sorokin, E. A. Vorob'eva, N. E. Suzina, V. I. Duda, V. F. Gal'chenko, G. I. El'-Registan, [Detection of microorganisms in the environment and the preliminary appraisal of their physiological state by X-ray microanalysis]. Microbiology. ,vol. 71, pp. 836- 848 ,(2002) , 10.1023/A:1021444309796
H. C. Bae, E. H. Cota-Robles, L. E. Casida, Microflora of Soil as Viewed by Transmission Electron Microscopy1 Applied Microbiology. ,vol. 23, pp. 637- 648 ,(1972) , 10.1128/AM.23.3.637-648.1972
Jennifer C Stern, Brad Sutter, Caroline Freissinet, Rafael Navarro-González, Christopher P McKay, P Douglas Archer Jr, Arnaud Buch, Anna E Brunner, Patrice Coll, Jennifer L Eigenbrode, Alberto G Fairen, Heather B Franz, Daniel P Glavin, Srishti Kashyap, Amy C McAdam, Douglas W Ming, Andrew Steele, Cyril Szopa, James J Wray, F Javier Martín-Torres, Maria-Paz Zorzano, Pamela G Conrad, Paul R Mahaffy, MSL Science Team, None, Evidence for indigenous nitrogen in sedimentary and aeolian deposits from the Curiosity rover investigations at Gale crater, Mars. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 112, pp. 4245- 4250 ,(2015) , 10.1073/PNAS.1420932112
A. A. Pavlov, G. Vasilyev, V. M. Ostryakov, A. K. Pavlov, P. Mahaffy, Degradation of the organic molecules in the shallow subsurface of Mars due to irradiation by cosmic rays Geophysical Research Letters. ,vol. 39, ,(2012) , 10.1029/2012GL052166
Pierre Amato, Shawn M. Doyle, John R. Battista, Brent C. Christner, Implications of subzero metabolic activity on long-term microbial survival in terrestrial and extraterrestrial permafrost. Astrobiology. ,vol. 10, pp. 789- 798 ,(2010) , 10.1089/AST.2010.0477
D.A. Gilichinsky, G.S. Wilson, E.I. Friedmann, C.P. Mckay, R.S. Sletten, E.M. Rivkina, T.A. Vishnivetskaya, L.G. Erokhina, N.E. Ivanushkina, G.A. Kochkina, V.A. Shcherbakova, V.S. Soina, E.V. Spirina, E.A. Vorobyova, D.G. Fyodorov-Davydov, B. Hallet, S.M. Ozerskaya, V.A. Sorokovikov, K.S. Laurinavichyus, A.V. Shatilovich, J.P. Chanton, V.E. Ostroumov, J.M. Tiedje, Microbial populations in Antarctic permafrost: biodiversity, state, age, and implication for astrobiology. Astrobiology. ,vol. 7, pp. 275- 311 ,(2007) , 10.1089/AST.2006.0012
T. O. Stevens, J. P. McKinley, Lithoautotrophic microbial ecosystems in deep basalt aquifers Science. ,vol. 270, pp. 450- 455 ,(1995) , 10.1126/SCIENCE.270.5235.450
J.D. Toner, D.C. Catling, B. Light, Soluble salts at the Phoenix Lander site, Mars: A reanalysis of the Wet Chemistry Laboratory data Geochimica et Cosmochimica Acta. ,vol. 136, pp. 142- 168 ,(2014) , 10.1016/J.GCA.2014.03.030
N. S. Duxbury, I. A. Zotikov, K. H. Nealson, V. E. Romanovsky, F. D. Carsey, A numerical model for an alternative origin of Lake Vostok and its exobiological implications for Mars Journal of Geophysical Research. ,vol. 106, pp. 1453- 1462 ,(2001) , 10.1029/2000JE001254
U. Böttger, J.-P. de Vera, J. Fritz, I. Weber, H.-W. Hübers, D. Schulze-Makuch, Optimizing the detection of carotene in cyanobacteria in a martian regolith analogue with a Raman spectrometer for the ExoMars mission Planetary and Space Science. ,vol. 60, pp. 356- 362 ,(2012) , 10.1016/J.PSS.2011.10.017