Loss of Colonic Structural Collagen Impairs Healing During Intra-abdominal Sepsis

摘要: Objective: To investigate colon anastomotic healing in an experimental model of sepsis. Design: Prospective, randomized, trial. Setting: Experimental surgical laboratory a large community hospital. Study Participants: Twenty-eight male Sprague-Dawley rats weighing 310 to 380 g. Interventions: On day 0, the underwent either sham laparotomy or cecal ligation and puncture. The next day, left resection single-layer inverted anastomosis. Colon-bursting pressure was determined 5 days after surgery at which time anastomosis segment 3 cm proximal were excised. Main Outcome Measures: pressure, colonic hydroxyproline concentration (index collagen content), total protein measured as α-amino nitrogen. Result: Sepsis resulted decreased bursting all segments that analyzed animals puncture compared with control animals. Conclusions: impairs colon, reflected by concentration. decrease bowel wall structural may affect ability gut hold sutures thus lead more failure. (Arch Surg. 1994;129:1179-1183)