Anti-RhoA and Anti-RhoC siRNAs Inhibit the Proliferation and Invasiveness of MDA-MB-231 Breast Cancer Cells in Vitro and in Vivo

摘要: Overexpression of RhoA or RhoC in breast cancer indicates a poor prognosis, due to increased tumor cell proliferation and invasion tumor-dependent angiogenesis. Until now, the strategy blockage Rho-signaling pathway has used either GGTI HMG-CoA reductase inhibitors, but they are not specific inhibition. In this study, new approach with anti-RhoA anti-RhoC siRNAs was inhibit specifically synthesis. Two transfections siRNA (8.5 nM) into MDA-MB-231 human cells HMEC-1 endothelial induced extensive degradation target mRNA led dramatic decrease synthesis corresponding protein. vitro, these inhibited more effectively than conventional blockers Rho signaling. Finally, nude mouse model, intratumoral injections (100 microl at 85 every 3 days for 20 almost totally growth angiogenesis xenografted tumors. One may infer from observations that inhibition represents promising treatment aggressive cancers.