Function and Expression Pattern of the α Subunit of the Heterotrimeric G Protein in Rice

摘要: The d1 mutant, which is deficient for the heterotrimeric G-protein alpha subunit (Galpha) gene of rice, shows dwarfism and sets small round seeds. To determine whether in due to a reduction cell number or shortened length, leaf sheath, internode, root lemma was compared between Nipponbare, wild-type rice d1-5, allele derived from Nipponbare. Our results indicate that reduced all organs analyzed d1-5. In addition, enlargement found roots although organ length d1-5 shorter than rice. These suggest Galpha participates proliferation Western blot analyses using anti-Galpha antibody RT-PCR mostly expressed developing organs. promoter activity studies GUS reporter confirmed expression highest We conclude regulation developmental stage-dependent manner.