作者: J E Heuser , M W Kirschner
DOI: 10.1083/JCB.86.1.212
关键词:
摘要: This report presents the appearance of rapidly frozen, freeze-dried cytoskeletons that have been rotary replicated with platinum and viewed in transmission electron microscope. The resolution this method is sufficient to visualize individual filaments cytoskeleton discriminate among actin, microtubules, intermediate solely by their surface substructure. identification has confirmed specific decoration antibodies selective extraction filament types, correlated light microscope immunocytochemistry gel electrophoresis patterns. freeze-drying preserves a remarkable degree three-dimensionality organization these cytoskeletons. They look strikingly similar meshwork strands or "microtrabeculae" seen cytoplasm whole cells high voltage microscopy, form lattice same configutation proportions open area as microtrabeculae cells. major differences between two views structural elements cytoplasmic matrix can be attributed effects aldehyde fixation dehydration. Freeze-dried thus provide an opportunity study--at absence problems caused chemical fixation--the detailed different regions at stages cell development. In pattern actin various fully spread mouse fibroblasts described.