Extraction of DNA by boiling cells in an alkaline phenol/guanidine thiocyanate solution

摘要: Nucleic acid probes and primers are described for detecting fungi that cause disease in humans animals, as well spoilage of food beverages. These can detect rRNA, rDNA or polymerase chain reaction products from a majority clinical, environmental samples. hybridization assay specific Acremonium sp., Aspergillus clavatus, flavus, fumigatus, glaucus, nidulans, niger, ochraceus, terreus, unguis, ustus, Beauveria Bipolaris Blastoschizomyces Blastomyces dermatitidis, Candida albicans, glabrata, guilliermondii, kefyr, krusei, lusitaniae, parapsilosis, tropicalis, Chrysosporium Cladosporium Coccidioides immitis, Cryptococcus neoformans var gattii serotype B, A, laurentii, Curvularia Fusarium Filobasidium capsuligenum, Filobasidiella (Cryptococcus) bacillispora C, D, uniguttulatum, Geotrichum Histoplasma capsulatum, Malbranchea Mucor Paecilomyces Penicillium species, Pseudallescheria boydii, Rhizopus Sporothrix schenkii, Scopulariopsis brevicaulis, brumpti, Saccharomyces cerevisiae, Trichosporon beigelii also described.