Impact of narrow-spectrum penicillin V on the oral and faecal resistome in a young child treated for otitis media

摘要: Abstract Objectives Antibiotic overuse has led to the global emergence of antimicrobial-resistant bacteria, and children are among most frequent users antibiotics. Most studies with broad-spectrum antibiotics show a severe impact on resistome development in patients. Although narrow-spectrum believed have fewer side effects, their microbiome is mostly unknown. The aim this study was investigate antibiotic phenoxymethylpenicillin (penicillin V) child treated for acute otitis media. Methods Oral faecal samples were collected from 1-year-old before (Day 0) after (Days 5 30) receiving penicillin V Metagenomic sequencing data analysed determine taxonomic profiling using Kraken Bracken software, resistance KMA combination ResFinder database. Results In oral samples, antimicrobial genes (ARGs) belonging four classes identified at baseline. At Day 5, abundance some ARGs increased, whereas remained unchanged others could no longer be detected. 30, had returned baseline levels or lower. seven observed five 5. number increased 21. Conclusions Following V, we remarkable enrichment aecal resistome, indicating that even may important consequences selecting more resistant microbiome.