Insulin-induced Glut4 recruitment in the fatty Zucker rat heart is not associated with changes in Glut4 content in the intracellular membrane

摘要: Impaired cardiac glucose metabolism and transport have been shown in the insulin resistant fatty Zucker rat. The aim of present study was to examine translocation insulin-sensitive transporter (Glut4) heart rat under vivo conditions. Insulin injected into both lean (FA/?) (fa/fa) rats via tail vein. time course Glut4 studied by determining subcellular distribution using a newly developed ELISA quantitation method. (10 U/kg) caused 30% 37% increase plasma membrane content at 20 min after injection respectively. contents basal insulin-stimulated states were significantly lower when compared control. dose effect (2.5-10 on mobilization similar phenotypes. (5-30 min), which rats, showed that maximal reached 5 post persisted throughout remaining 25 min. However, intracellular remained unchanged all doses points studied. Collectively, these results show recruitment is responsive response rats. not associated with changes content.