Formation of monoclonal antibody against a major ginseng component, ginsenoside Rb1 and its characterization.
作者： Hiroyuki Tanaka , Noriko Fukuda , Yukihiro Shoyama
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摘要: The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rb1 produced fusing splenocytes immunized with a Rb1- HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. very small cross-reaction appeared Rc Rd. full measuring range the assay extends from 20 ngml-1 to 400 Rb1.
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