Concerning the mechanism of complement action. IV. The properties of activated first component of guinea pig complement.

摘要: Summary C′1 and TAMe esterase activity can be eluted from sensitized sheep red cells containing by ethylenediaminetetraacetic acid. On dialyzing the supernatant so treated against a low ionic strength buffer, both activities recovered. The in this fashion detected with case two-step procedure of adding “eluate” to cells, washing then an R1. If R1, are added together little or no evidence for is obtained. properties enzyme when removed cell same far as studied attached cell. When eluate not only does attach itself but well. Allowing stand cold at pH 3.3, affects its all; however, ability destroyed and, kinetic studies showed, extent. In explanation these latter observations hypothesis was evoked that molecule contains two distinct sites, binding site enzymatic site, required order manifest activity.