Porcine endogenous retrovirus encodes xenoantigens involved in porcine cellular xenograft rejection by mice.

摘要: Background. Identification of the antigens that stimulate transplant rejection can help develop graft-specific antirejection strategies. The xenoantigens recognized during porcine cellular xenografts have not been clearly defined, but it has assumed major histocompatibility complex (MHC) are involved. Methods. role endogenous retrovirus (PERV) as a source was examined. authors used morphometry to compare kinetics swine leukocyte antigen (SLA) dd pig thyroid xenograft in control mice and immunized with PERV + PK15 cells (porcine kidney epithelial cells), - SLA peripheral blood lymphocytes (PBL), virions purified from cells, or A pseudotypes produced infected human 293 cells. tempo for pseudotype-producing uninfected PERV-preimmunized also compared. Results. Mice rejected significantly faster at day 5 than PBL. This correlated amount RNA produced, class I MHC expressed by Immunization induced accelerated days. Accelerated virus pretreatment CD4 T-cell dependent restricted PERV-expressing nonporcine origin. Conclusions. acts significant target rejection.