Reduced Expression of P2Y1 Receptors in Connexin43-Null Mice Alters Calcium Signaling and Migration of Neural Progenitor Cells

摘要: Glial calcium signals play important roles during CNS development. Calcium transients induced by ATP, acting on purinergic receptors, stimulate DNA synthesis, increase astrocytic and neural stem cell proliferation, are prominent the differentiation of radial glia. We have shown previously that expression P2Y receptors in astrocytes is altered when connexin43 (Cx43) downregulated. To evaluate consequences Cx43 deletion signaling progenitor development, studies were performed neurospheres derived from embryonic striatum. After adhesion, cells migrating wild-type (WT) Cx43-null displayed spontaneous oscillations. Such activity was blunted apyrase, 2′-deoxy- N 6 -methyladenosine 3′,5′-bisphosphate (MRS-2179), suramin, suggesting ATP released acts to induce The amplitudes Ca 2+ but not P2X receptor agonists larger WT than progenitors, these two populations express different P2 receptors. Suramin, a nonselective antagonist, MRS-2179, 1 receptor-selective reduced proliferation rate migration levels similar those cells. Conversely, exogenous restored their pattern seen progenitors. However, treatment with antagonists did alter ratio nestin GFAP These data show autocrine-paracrine communication attributable lacking affects early