Successful Clinical Implementation of Corneal Epithelial Stem Cell Therapy for Treatment of Unilateral Limbal Stem Cell Deficiency

摘要: The corneal epithelium is maintained by a population of stem cells known as limbal (LSCs) due to their location in the basal layer outer border cornea limbus. Treatment cell deficiency (LSCD) has been achieved with transplantation ex vivo expanded LSCs taken from small biopsy This relatively new technique, and such, specific national or international guidance yet be established. Because lack such guidance, our group sought minimize any risk patient adopting certain modifications research methodologies use at present. These include replacement all non-human animal products culture system production reagents cultures under Good Manufacturing Practice conditions. In addition, for first time, strictly defined uniform patients total unilateral LSCD no other significant ocular conditions used allow success failure treating attributable directly proposed therapy. A prospectively designed study strict inclusion exclusion criteria was enroll database LSCD. Eight eyes eight consecutive treated autologous LSC transplant on human amniotic membrane (HAM) mean follow-up 19 (RANGE) months were included study. Postoperatively, satisfactory surface reconstruction stable obtained (100%). At last examination, best corrected visual acuity improved five remained unchanged three eyes. Vision impairment pain scores (p < .05). demonstrates that epithelial cultured HAM without safe effective method reconstructing restoring useful vision