Alteration of a yeast SH3 protein leads to conditional viability with defects in cytoskeletal and budding patterns.

摘要: Mutations in genes necessary for survival stationary phase were isolated to understand the ability of wild-type Saccharomyces cerevisiae remain viable during prolonged periods nutritional deprivation. Here we report results concerning one these mutants, rvs167, which shows reduced viability and abnormal cell morphology upon carbon nitrogen starvation. The mutant exhibits same response when cells are grown high salt concentrations other unfavorable growth conditions. RVS167 gene product displays significant homology with Rvs161 protein contains a SH3 domain at C-terminal end. Abnormal actin distribution is associated phenotype. In addition, while budding pattern haploid strains remains axial standard conditions, diploid random. therefore could be implicated cytoskeletal reorganization environmental stresses act site selection mechanism.