A single-chain antibody fragment against human thyroglobulin: construction and evaluation of immunoreactivity.
作者： Rekha Rajawat , Archana Narkar , Archana Damle , G.B. Sunil Kumar , K.P. Mishra
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摘要: Smaller recombinant antibody fragments are at the forefront of in vivo diagnosis and therapy. These units possess better distribution faster clearance than larger molecules. Among these, single chain (scFv) emerging as credible alternatives. proteins shown to have same specificities affinities for their antigens parental monoclonal (MAb). We attempted produce scFv against human thyroglobulin (H-Tg) using anti-Tg secreting hybridoma cells PCR-based cloning approach. Hybridoma MAb B10IV was established. cDNA prepared from cells. The VH VL genes were amplified cloned. gene sequences submitted Genebank database (accession nos. AJ508533 AM072962, respectively.) then linked together with a linker peptide successfully cloned pET28a expressed His-tag fusion protein expression host BL21 (DE3). IPTG-induced purified under n...
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