Chronic prenatal ethanol exposure and increased concentration of fatty acid ethyl esters in meconium of term fetal Guinea pig.

摘要: In humans, the occurrence of prenatal exposure to ethanol is difficult validate objectively. Increased concentration fatty acid ethyl esters (FAEE) in meconium newborn may be a biomarker exposure. The validity this proposed was tested pregnant guinea pigs that received chronic oral administration 4 g ethanol/kg maternal body weight/day (n=8), isocaloric-sucrose/pair-feeding (n=8) or water (n=2) throughout gestation. At gestational day 65 (term, 66 69), each dam and her offspring were euthanized, collected from term fetal large intestine. Eight individual FAEE (lauric, myristic, palmitic, palmitoleic, stearic, oleic, linolenic arachidonic AEE) measured by gas chromatography--flame ionization detection confirmed chromatography--mass spectrometry. regimen decreased weight brain weight. There virtually no measurable for group (n=3 fetuses). For (n=25 fetuses) compared with sucrose (n=23 fetuses), total 8-fold higher; lauric, stearic oleic AEE concentrations at least 5-fold higher group. data indicate constitute restricts development, an inverse relationship between both