Identification of MINUS, a small polypeptide that functions as a microtubule nucleation suppressor
作者： Patrizia Fanara , Björn Oback , Keith Ashman , Alexandre Podtelejnikov , Roland Brandt
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摘要: In eukaryotic cells, tubulin polymerization must be regulated precisely during cell division and differentiation. To identify new mechanisms involved in cellular microtubule formation, we isolated an activity that suppresses nucleation vitro. The was due to a small acidic polypeptide of 4.7 kDa which named MINUS (microtubule suppressor). inhibited tau- taxol-mediated assembly vitro inactivated by dephosphorylation. protein purified homogeneity from cultured neural (PC12) cells bovine brain. Microinjection caused transient loss dynamic microtubules Vero cells. results suggest acts with novel mechanism on polymerization, thus regulating formation living
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W A Voter, H P Erickson, The kinetics of microtubule assembly. Evidence for a two-stage nucleation mechanism. Journal of Biological Chemistry. ,vol. 259, pp. 10430- 10438 ,(1984) , 10.1016/S0021-9258(18)90982-8
R. Brandt, G. Lee, Functional organization of microtubule-associated protein tau. Identification of regions which affect microtubule growth, nucleation, and bundle formation in vitro. Journal of Biological Chemistry. ,vol. 268, pp. 3414- 3419 ,(1993) , 10.1016/S0021-9258(18)53710-8
Lars Sundberg, Jerker Porath, Preparation of adsorbents for biospecific affinity chromatography. Attachment of group-containing ligands to insoluble polymers by means of bifuctional oxiranes. Journal of Chromatography A. ,vol. 90, pp. 87- 98 ,(1974) , 10.1016/S0021-9673(01)94777-6
Susumu KOTANI, Atsushi IKAI, Gota KAWAI, Shohei MAEKAWA, Shigeyuki YOKOYAMA, Hikoichi SAKAI, Microtubule-assembly inhibitor protein FEBS Journal. ,vol. 176, pp. 573- 580 ,(1988) , 10.1111/J.1432-1033.1988.TB14316.X
N. Kumar, Taxol-induced polymerization of purified tubulin. Mechanism of action. Journal of Biological Chemistry. ,vol. 256, pp. 10435- 10441 ,(1981) , 10.1016/S0021-9258(19)68639-4
T. E. Kreis, Microtubules containing detyrosinated tubulin are less dynamic. The EMBO Journal. ,vol. 6, pp. 2597- 2606 ,(1987) , 10.1002/J.1460-2075.1987.TB02550.X
PH O'Farrell, High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry. ,vol. 250, pp. 4007- 4021 ,(1975) , 10.1016/S0021-9258(19)41496-8
G. Lee, S.L. Rook, Expression of tau protein in non-neuronal cells: microtubule binding and stabilization. Journal of Cell Science. ,vol. 102, pp. 227- 237 ,(1992) , 10.1242/JCS.102.2.227
Francis J McNally, Modulation of microtubule dynamics during the cell cycle Current Opinion in Cell Biology. ,vol. 8, pp. 23- 29 ,(1996) , 10.1016/S0955-0674(96)80044-5
Tim Mitchison, Marc Kirschner, Dynamic instability of microtubule growth Nature. ,vol. 312, pp. 237- 242 ,(1984) , 10.1038/312237A0