Cloning and functional expression of a novel long-chain acyl-CoA synthetase expressed in brain.

摘要: Complementary DNA clones encoding a protein highly homologous to the previously characterized long-chain acyl-CoA synthetase (LACS) in liver were isolated from rat brain cDNA libraries. This consists of 697 amino acids and has 64.7% identity with LACS sequence. The share essentially same domain structure, having two regions similar those click beetle luciferase long discrete gap flanking domains. A significant sequence similarity was found between malaria octapeptide-repeat antigen, suggesting functional similarity. COS cells transfected for expressed activity slightly different fatty acid specificity that LACS. new is predominantly and, much lesser extent, heart adrenal. 2.9- 6.3-kb mRNAs coding enzyme are coregulated development brain, physiological importance metabolism brain.