Differential responses of scirrhous and well-differentiated gastric cancer cells to orthotopic fibroblasts.

摘要: Scirrhous gastric cancer cells proliferate rapidly with fibrosis, when the invade into submucosa of stomach. To investigate mechanisms responsible for rapid proliferation, growth interaction between and fibroblasts was examined. Human cell lines established from scirrhous carcinoma or well-differentiated adenocarcinoma were used. fibroblast obtained various organs. The examined by calculating number measuring [3H]thymidine incorporation cells. Gastric specifically stimulated cells, but not that factor(s) produced then partially purified characterised. growth-promoting had apparent molecular weights 10000 dalton sensitive both to heat proteinase treatment. No inhibition achieved defined anti-growth factor antibodies. In this study, differential responses orthotopic shown. Rapid proliferation should be partly controlled fibroblasts. fibroblasts, which distinct factors such as epidermal (EGF), basic (b-FGF), transforming factor-alpha (TGF-alpha), keratinocyte (KGF), vascular endothelial (VEGF), insulin-like I (IGF-I), hepatocyte (HGF), platelet-derived (PDGF) beta 1 (TGF-beta 1) may play an important role in progression