Role for interleukin‐4 in foreign‐body giant cell formation on a poly(etherurethane urea) in vivo

摘要: Interleukin-4 (IL-4) was previously shown to induce extensive macrophage fusion form foreign-body giant cells (FBGCs) in vitro. In the present study, our goal extend these findings an vivo test environment on biomaterials. The subcutaneous cage-implant system modified for mice elucidate IL-4 participation mediating FBGC formation vivo. Exudate leukocyte concentrations from cages containing poly(etherurethane urea) (PEUU A') and empty cage controls indicated a similar inflammatory response that turned toward resolution by 14 days postimplantation, thus confirming applicability of mice. kinetic analysis showed mouse FBGCs occurs through adherent macrophages at constant rate up implantation. Purified goat anti-mouse neutralizing antibody (IL4Ab) or normal nonspecific control IgG (gtIgG) various concentrations, recombinant murine (muIL4) injected into implanted PEUU A' every 2 7 days. injection IL4Ab significantly decreased density cage-implanted mice, when compared with PBS controls. Conversely, increased muIL4 Adherent density, morphology, average size, size distribution were not different among IL4Ab, gtIgG, muIL4, groups. Our data suggest participates biomaterials