[12] Migration of DNA through gels

摘要: Publisher Summary Gel electrophoresis has become a major laboratory tool for separating biological macromolecules. For example, large (megabase) double-stranded DNA (dsDNA) molecules can readily be separated on agarose gels, using pulsed-field gel (PFGE), while subkilobase single-stranded (ssDNA) sequenced polyacrylamide gels. These two techniques are essential to map and sequence the human genome. This chapter first discusses different models of migration through then their limitations conditions, under which they should apply. It describes how experimental data obtained analyzed in order (1) identify mechanism that is taking place, (2) estimate parameters relevant model, (3) optimize separation. As an it applies this approach separation (sequencing) ssDNA concludes, by briefly reviewing challenges still open study sequencing, introduces some new ideas being explored.