Constitutive Expression of the HTLV-I pX and env Regions in Jurkat T-cells Induces Differential Activation of SRE, CRE and NFκB Pathways
作者： Alexander C. Black , Jie Luo , Susan Chun , Sepehr Tabibzadeh
关键词:
摘要: Human T-cell leukemia virus type I (HTLV-I) causes adult leukemia/lymphoma (ATLL). HTLV Tax, the viral transcriptional activator, can activate a variety of cellular genes. HTLV-mediated transformation, however, may involve additional proteins expressed from singly- as well doubly-spliced mRNA. To determine combined effect these on gene expression in Jurkat T-cells, we derived stable transfectants that constitutively express HTLV-I pX and env regions (J3.9). J3.9 cells show substantially increased mRNA levels egr-1 c-jun but no induction either CD25 or GM-CSF by Northern blotting. This pattern corresponded to activation an not promoter-driven reporter construct transient assays. In DNA electrophoretic mobility shift assays (EMSA), nuclear extract has significantly binding CRE SRE factor kappa B (NFκB) oligos, compared J-Neo cell extract. These results suggest low level region products T-cells stimulates persistent CRE- SRE- NFκB-induced
springer.com
sci-hub.se 参考文章(47)
L.C. Sambucetti, J.M. Cherrington, G.W. Wilkinson, E.S. Mocarski, NF-kappa B activation of the cytomegalovirus enhancer is mediated by a viral transactivator and by T cell stimulation. The EMBO Journal. ,vol. 8, pp. 4251- 4258 ,(1989) , 10.1002/J.1460-2075.1989.TB08610.X
C C Li, F W Ruscetti, N R Rice, E Chen, N S Yang, J Mikovits, D L Longo, Differential expression of rel family members in human T-cell leukemia virus type I-infected cells : transcriptional activation of c-rel by tax protein Journal of Virology. ,vol. 67, pp. 4205- 4213 ,(1993) , 10.1128/JVI.67.7.4205-4213.1993
Y Furukawa, M Osame, R Kubota, M Tara, M Yoshida, Human T-cell leukemia virus type-1 (HTLV-1) tax is expressed at the same level in infected cells of HTLV-1- associated myelopathy or tropical spastic paraparesis patients as in asymptomatic carriers but at a lower level in adult T-cell leukemia cells Blood. ,vol. 85, pp. 1865- 1870 ,(1995) , 10.1182/BLOOD.V85.7.1865.BLOODJOURNAL8571865
S R Himes, R Katsikeros, M F Shannon, Costimulation of cytokine gene expression in T cells by the human T leukemia/lymphotropic virus type 1 trans activator Tax. Journal of Virology. ,vol. 70, pp. 4001- 4008 ,(1996) , 10.1128/JVI.70.6.4001-4008.1996
E Muñoz, G Courtois, P Veschambre, P Jalinot, A Israël, Tax induces nuclear translocation of NF-kappa B through dissociation of cytoplasmic complexes containing p105 or p100 but does not induce degradation of I kappa B alpha/MAD3. Journal of Virology. ,vol. 68, pp. 8035- 8044 ,(1994) , 10.1128/JVI.68.12.8035-8044.1994
N Arima, J A Molitor, M R Smith, J H Kim, Y Daitoku, W C Greene, Human T-cell leukemia virus type I Tax induces expression of the Rel-related family of kappa B enhancer-binding proteins: evidence for a pretranslational component of regulation. Journal of Virology. ,vol. 65, pp. 6892- 6899 ,(1991) , 10.1128/JVI.65.12.6892-6899.1991
J Lacoste, L Petropoulos, N Pépin, J Hiscott, Constitutive phosphorylation and turnover of I kappa B alpha in human T-cell leukemia virus type I-infected and Tax-expressing T cells. Journal of Virology. ,vol. 69, pp. 564- 569 ,(1995) , 10.1128/JVI.69.1.564-569.1995
T Akagi, K Shimotohno, Proliferative response of Tax1-transduced primary human T cells to anti-CD3 antibody stimulation by an interleukin-2-independent pathway. Journal of Virology. ,vol. 67, pp. 1211- 1217 ,(1993) , 10.1128/JVI.67.3.1211-1217.1993
T Akagi, H Ono, K Shimotohno, Characterization of T Cells Immortalized by Tax1 of Human T-Cell Leukemia Virus Type 1 Blood. ,vol. 86, pp. 4243- 4249 ,(1995) , 10.1182/BLOOD.V86.11.4243.BLOODJOURNAL86114243
G Franchini, Molecular mechanisms of human T-cell leukemia/lymphotropic virus type I infection. Blood. ,vol. 86, pp. 3619- 3639 ,(1995) , 10.1182/BLOOD.V86.10.3619.BLOODJOURNAL86103619