Intracellular responses of identified rat olfactory bulb interneurons to electrical and odor stimulation.

摘要: 1. Intracellular recordings were made from 28 granule cells and 6 periglomerular of the rat olfactory bulb during odor stimulation electrical nerve layer (ONL) lateral tract (LOT). Neurons identified by injection horseradish peroxidase (HRP) or biocytin and/or intracellular response characteristics. Odorants presented in a cyclic sniff paradigm, as reported previously. 2. All interneurons could be activated wide number sites on ONL, with distances exceeding their known dendritic spreads dispersion fibers within indicating that multisynaptic pathways must also exist at glomerular region. types responded to odorant stimulation, showing variety responses. 3. Granule LOT ONL However, potential, excitability, conductance analysis suggested mitral cell-mediated excitatory postsynaptic potential (EPSP) is followed long inhibitory (IPSP). An early negative before EPSP, was observed every cell correlated component I extracellular LOT-induced field potential. We have interpreted this negativity "field effect," may diagnostic cells. 4. Most exhibited responses stimulation. Odors produce spiking either nonhabituating (response sniff) rapidly habituating first only). Other cells, while showed depolarizations did not evoke spikes These transient each sustained across series sniffs. physiological differences position beneath for 12 suggesting morphological subtypes show differences. Some features seem correlate some we mitral/tufted recordings. Only one inhibition odors. 5. Periglomerular (PG) unlike found other neurons. There long-duration hyperpolarization after spike large depolarization burst (20-30 ms duration). Odor produced simple bursts action potentials, PG simply follow input nerve.(ABSTRACT TRUNCATED AT 400 WORDS)