Simultaneous silencing of endo-β-1,4 xylanase genes reveals their roles in the virulence of Magnaporthe oryzae.

摘要: Due to functional redundancy, it is often difficult genetically analyse the biological function of fungal cell wall-degrading enzymes that belong a gene family. To overcome this difficulty, we used RNAi knock-down (KD) multiple xylanase genes elucidate their roles in pathogenicity blast fungus, Magnaporthe oryzae. obtain maximum average efficiency silencing for genes, 'building blocks method', which 40 bp sequence was chosen from an endoxylanase gene, and 10 such sequences endoxylanases were combined make artificial trigger by synthetic DNA. Quantitative RT-PCR analysis revealed transcript levels all expressed significantly reduced KD mutants with trigger. Even though did not completely lose host plants, number lesions, rate penetration extent infected cells mutant-infected leaves. The degree reduction associated mRNA enzymatic activity mutants. Cytological indicated xylanases play significant both vertical horizontal expansion M. oryzae plants.