RECEPTOR PROTEIN TYROSINE PHOSPHATASES ARE EXPRESSED BY CYCLING RETINAL PROGENITOR CELLS AND INVOLVED IN NEURONAL DEVELOPMENT OF MOUSE RETINA

摘要: Receptor protein tyrosine phosphatases (RPTPs) appear to coordinate many aspects of neural development, including cell proliferation, migration and differentiation. Here we investigated potential roles RPTPs in the developing mouse retina. Using a degenerate oligonucleotide-based reverse transcription polymerase chain reaction approach, identified 11 different retina at embryonic stage 13 (E13). Subsequently, expression patterns RPTPkappa, RPTPJ, RPTPRR, RPTPsigma, RPTPepsilon RPTPgamma from stages adult were analyzed detail using quantitative real-time-PCR, situ hybridization, immunohistochemistry Western blotting. At E13, all six are expressed actively cycling retinal progenitor cells postmitotic newborn neurons. With ongoing maturation, display spatiotemporal regulation mRNAs proteins pre- postnatal Finally, adulthood these localize distinct cellular compartments multiple Additional studies RPTPgamma(-/-) RPTPbeta/zeta(-/-) (also known as PTPRZ1, RPTPbeta or RPTPzeta) mice P1 reveal no apparent differences laminar organization pattern specific cell-type markers when compared with wild type. However, retinas, immunoreactivity vimentin, marker Muller glial cells, is selectively reduced morphology vimentin-immunoreactive radial processes considerably disturbed. Our results suggest proliferation establishing phenotypes during retinogenesis well later maintenance mature