Immunosensor methods for drug residue control of food

摘要: The use of drugs to improve animal growth is banned in the European Union. Nevertheless, illegal has been demonstrated several countries and Member States have run extensive control programs ensure that ban respected. aim this work was explore possibility using surface plasmon resonance (SPR) biosensors develop fast robust screening assays for hormones s-agonists. Modified standard procedures hapten synthesis were employed immobilise stable analyte specific sensor chip surfaces used analysis. Sensor chips clenbuterol, ethinylestradiol trenbolone gave with IC50 values region 0.5 ng/ml. Sample matrix interference from urine serum antibody-antigen interaction non-specific binding samples systematically studied. Strategies minimise these effects investigated described. Biosensor clenbuterol bovine hair urine, suitable routine use, developed validated. For hair, a sandwich assay format used. A rapid simple extraction 100 mM NaOH developed, validated by analysis animals treated clenbuterol. critical factor sensitivity extracts, which controlled ultrafiltration secondary antibody. limit detection (LOD) 10 In assay, cross-reaction s-agonists, including salbutamol, mabuterol brombuterol high, enabling concentrations below 1 Analysis low without sample pre-treatment limited high sample-to-sample variation, inhibition antibody occasional binding. clean-up integrated immunofiltration effects. With method, at least 48 could be processed analysed one person working day.