Cellular Response to Sol-Gel Hybrid Materials Releasing Boron and Calcium Ions.

摘要: Poly(dimethylsiloxane) (PDMS)-SiO2-CaO-based hybrid materials prepared by sol-gel have proved to be very promising for tissue engineering applications and drug-delivery systems. These hybrids are biocompatible present osteogenic bioactive properties supporting osteoblast attachment bone growth. The incorporation of therapeutic elements in these materials, such as boron (B) calcium (Ca), was considered this study an approach develop biomaterials capable stimulating regeneration. main purpose work thus produce, sol-gel, the PDMS-SiO2-B2O3-CaO system, a controlled Ca B release. Different compositions with different amounts were using same precursors resulting monolithic distinct structures microstructures. Structural features assessed Fourier transform infrared (FT-IR) spectrometry solid-state nuclear magnetic resonance (NMR) techniques, which confirmed presence bonds (Si-O-Si) between organic (PDMS) inorganic phase (tetraethyl orthosilicate (TEOS)), well borosiloxane (B-O-Si). From 11B NMR results, it found that changes coordination, from trigonal (BO3) tetrahedral (BO4). Scanning electron microscopy (SEM) micrographs N2 isotherms showed modifies material's microstructure increasing macroporosity decreasing specific surface area (SSA). In vitro tests simulated body fluid (SBF) precipitation phosphate layer on material release ions. cytocompatibility studied marrow stromal cells (ST-2 cell line) analyzing viability density. results demonstrated dilution rate extraction medium leads improved behavior. relationship response structural microstructural explored. It shown ions, determined structure crucial observed Although not completely understood, encouraging obtained constitute incentive further studies topic.