Matrix Metalloprotease 2 (MMP-2) and Matrix Metalloprotease 9 (MMP-9) Type IV Collagenases in Colorectal Cancer

摘要: Using quantitative zymography, we measured activity of the type IV collagenases metalloprotease 2 (MMP-2) and MMP-9 in 192 biopsies from colorectal carcinomas, adenomas, normal bowel. The median level samples Dukes' stage A (n = 18) or C 48) tumors was significantly higher than B carcinomas 65), adenomas 25), normals 36; P 0.0001). active MMP-2 compared with (P 0.0001) inactive all stages There a significant increase Jass prognostic groups I-IV 0.006) but no correlation enzyme. MMP not related to tumor differentiation, colon versus rectal location, disease-free, 5-year survival. All expressed mRNA for both enzymes, there were locational differences expression between normal, benign, malignant tissues. Thus is controlled at protein production activation cancer, enzymes are associated strongly disease. Variations levels group cancer reflect their differing stromal content.