Control of rRNA synthesis in Escherichia coli at increased rrn gene dosage. Role of guanosine tetraphosphate and ribosome feedback.
作者： E. Baracchini , H. Bremer
DOI: 10.1016/S0021-9258(18)99021-6
关键词:
摘要: Abstract The effects of extra, plasmid-borne rRNA genes on the synthesis rate in Escherichia coli were examined by measuring fraction total RNA that is and tRNA (rs/rt), cytoplasmic concentration guanosine tetraphosphate (ppGpp), absolute rates protein synthesis. Experiments carried out different growth media with two strains E. coli, B/r K-12. results indicated: 1) increased rrn gene dosage from either intact or defective reduced bacterial ribosome activity (protein rate/average ribosome), rs/rt. 2) Extra intact, but not extra defective, caused level ppGpp to be comparison pBR322-carrying control strain. 3) As a function ppGpp, rs/rt was genes. 4) rate/rrn presence genes; this reduction greater than An analysis these showed they are consistent hypothesis feedback effector role translating ribosomes.
sci-hub.st 参考文章(29)
J.R. Cole, C.L. Olsson, J.W.B. Hershey, M. Grunberg-Manago, M. Nomura, Feedback regulation of rRNA synthesis in Escherichia coli. Requirement for initiation factor IF2. Journal of Molecular Biology. ,vol. 198, pp. 383- 392 ,(1987) , 10.1016/0022-2836(87)90288-9
R Little, J Ryals, H Bremer, rpoB mutation in Escherichia coli alters control of ribosome synthesis by guanosine tetraphosphate. Journal of Bacteriology. ,vol. 154, pp. 787- 792 ,(1983) , 10.1128/JB.154.2.787-792.1983
R R Dickson, T Gaal, H A deBoer, P L deHaseth, R L Gourse, Identification of promoter mutants defective in growth-rate-dependent regulation of rRNA transcription in Escherichia coli. Journal of Bacteriology. ,vol. 171, pp. 4862- 4870 ,(1989) , 10.1128/JB.171.9.4862-4870.1989
Patrick P. Dennis, Stable Ribonucleic Acid Synthesis during the Cell Division Cycle in Slowly Growing Escherichia coli B/r Journal of Biological Chemistry. ,vol. 247, pp. 204- 208 ,(1972) , 10.1016/S0021-9258(19)45776-1
E Baracchini, H Bremer, Stringent and growth control of rRNA synthesis in Escherichia coli are both mediated by ppGpp. Journal of Biological Chemistry. ,vol. 263, pp. 2597- 2602 ,(1988) , 10.1016/S0021-9258(18)69108-2
R Little, H Bremer, Transcription of ribosomal component genes and lac in a relA+/relA pair of Escherichia coli strains. Journal of Bacteriology. ,vol. 159, pp. 863- 869 ,(1984) , 10.1128/JB.159.3.863-869.1984
V J Hernandez, H Bremer, Escherichia coli ppGpp synthetase II activity requires spoT. Journal of Biological Chemistry. ,vol. 266, pp. 5991- 5999 ,(1991) , 10.1016/S0021-9258(19)67695-7
H Xiao, M Kalman, K Ikehara, S Zemel, G Glaser, M Cashel, Residual guanosine 3',5'-bispyrophosphate synthetic activity of relA null mutants can be eliminated by spoT null mutations. Journal of Biological Chemistry. ,vol. 266, pp. 5980- 5990 ,(1991) , 10.1016/S0021-9258(19)67694-5
G Churchward, H Bremer, R Young, Transcription in bacteria at different DNA concentrations. Journal of Bacteriology. ,vol. 150, pp. 572- 581 ,(1982) , 10.1128/JB.150.2.572-581.1982
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)