Analysis of Lymphocytes, Monocytes, and Neutrophils from Human Immunodeficiency Virus (HIV)-Infected Persons for HIV DNA
作者： H. A. Kessler , J. L. Moore , G. Schochetman , A. L. Landay , G. T. Spear
DOI: 10.1093/INFDIS/162.6.1239
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摘要: The human immunodeficiency virus (HIV) infects and depletes or alters the function of cells involved in immune responsiveness. While both T helper lymphocytes monocyte/macrophages can be infected via cell-surface CD4 vitro, previous studies showed that few blood express HIV RNA vivo. This study used DNA amplification to determine levels purified lymphocytes, monocytes, neutrophils from HIV-infected asymptomatic individuals persons with AIDS. average numbers copies AIDS patients were similar (approximately 100-140 copies/150,000 cells). However, when expressed on basis CD4+ cells, patients' contained approximately 2.5 times more DNA. was present all 27 subjects, little no observed monocytes neutrophils. Only 1 person (125 proviral cells) comparable (160/150,000). expression monocyte this persistent over at least 8 months, it not neutrophils, suggesting did originate myeloid precursors. shows infection, are predominant cell found blood.
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M. E. Harper, L. M. Marselle, R. C. Gallo, F. Wong-Staal, Detection of lymphocytes expressing human T-lymphotropic virus type III in lymph nodes and peripheral blood from infected individuals by in situ hybridization Proceedings of the National Academy of Sciences of the United States of America. ,vol. 83, pp. 772- 776 ,(1986) , 10.1073/PNAS.83.3.772
Paul R. Clapham, Jonathan N. Weber, Denise Whitby, Kenneth McIntosh, Angus G. Dalgleish, Paul J. Maddon, Keith C. Deen, Raymond W. Sweet, Robin A. Weiss, Soluble CD4 blocks the infectivity of diverse strains of HIV and SIV for T cells and monocytes but not for brain and muscle cells Nature. ,vol. 337, pp. 368- 370 ,(1989) , 10.1038/337368A0
A Takeda, C. Tuazon, F. Ennis, Antibody-enhanced infection by HIV-1 via Fc receptor-mediated entry Science. ,vol. 242, pp. 580- 583 ,(1988) , 10.1126/SCIENCE.2972065
Mikulas Popovic, Suzanne Gartner, Isolation of HIV-1 from monocytes but not T lymphocytes. The Lancet. ,vol. 330, pp. 916- ,(1987) , 10.1016/S0140-6736(87)91403-6
Ferenc Gyorkey, JosephL. Melnick, JosephG. Sinkovics, Phyllis Gyorkey, Retrovirus resembling HTLV in macrophages of patients with AIDS. The Lancet. ,vol. 325, pp. 106- ,(1985) , 10.1016/S0140-6736(85)91995-6
T. Folks, S. Kessler, J. Orenstein, J. Justement, E. Jaffe, A. Fauci, Infection and replication of HIV-1 in purified progenitor cells of normal human bone marrow Science. ,vol. 242, pp. 919- 922 ,(1988) , 10.1126/SCIENCE.2460922
Agnès Cordonnier, Luc Montagnier, Michael Emerman, Single amino-acid changes in HIV envelope affect viral tropism and receptor binding. Nature. ,vol. 340, pp. 571- 574 ,(1989) , 10.1038/340571A0
S Koenig, H. Gendelman, J. Orenstein, M. Dal Canto, G. Pezeshkpour, M Yungbluth, F Janotta, A Aksamit, M. Martin, A. Fauci, Detection of AIDS virus in macrophages in brain tissue from AIDS patients with encephalopathy Science. ,vol. 233, pp. 1089- 1093 ,(1986) , 10.1126/SCIENCE.3016903
Anneka Ehrnst, A. Sönnerborg, S. Bergdahl, Ö. Strannegård, Efficient isolation of HIV from plasma during different stages of HIV infection Journal of Medical Virology. ,vol. 26, pp. 23- 32 ,(1988) , 10.1002/JMV.1890260105
C. Ou, S Kwok, S. Mitchell, D. Mack, J. Sninsky, J. Krebs, P Feorino, D Warfield, G Schochetman, DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells Science. ,vol. 239, pp. 295- 297 ,(1988) , 10.1126/SCIENCE.3336784