Lipopolysaccharide interaction with hemolin, an insect member of the Ig-superfamily

摘要: This study is an attempt to reach some understanding of how insects recognize intruding microorganisms and foreign entities while executing immune response. We used lipopolysaccharide (LPS) from Escherichia coli, bound a radiolabeled iodinated crosslinker, identify hemolymph proteins the Hyalophora cecropia moth that have capacity bind LPS. High amounts radioactivity were conferred hemolin, immunoglobulin NCAM-related protein, concentration which increases in insect upon bacterial infection. could demonstrate concentration-dependant binding hemolin In addition we show Lipid A can compete for this binding, whereas KDO has no effect, indicating interacts specifically with moiety