An evaluation of Irish cattle herds with inconclusive serological evidence of bovine brucellosis

作者: M Hayes , S Ashe , DM Collins , S Power , K Kenny

DOI: 10.1186/2046-0481-62-3-182

关键词:

摘要: Since 1998, there has been a steady decline in herd restrictions and de-populations Ireland due to bovine brucellosis. There is concern that the interpretation of laboratory results may become increasingly problematic, as brucellosis prevalence falls Ireland. Therefore, purpose current study was evaluate infection status Irish herds animals with inconclusive serological evidence During 12 months from September 1, 2004, observational epidemiological data were collected all where animal testing identified at least one complement fixation test (CFT) reading greater than zero and/or positive result indirect enzyme-linked immunosorbent assay (iELISA). Due nature study, we have robust estimates relative, but not absolute, performance CFT, iELISA brucellin skin (BST). Herds divided into three categories (Group A, B or C) on basis initial assessment. A total 639 enrolled observed for two years following enrolment. rising CFT titre, 111 International Units (IU) subsequent blood test, generally associated other also available. Knowledge proved useful distinguishing false-positive true-positive results. poor correlation between results, BST As this national policy modified include re-sampling readings 20 IU greater. This project led reduction number restricted, well restriction duration. It contributed listed contiguous tests, therefore potential contiguity false

参考文章(12)
R. A. Brewer, F. A. Stuart, M. J. Corbel, Observations on serological cross-reactions between smooth Brucella species and organisms of other genera. Developments in biological standardization. ,vol. 56, pp. 341- 348 ,(1984)
B Garin-Bastuji, G Gerbier, R Pouillot, Y Coche, C Cau, B Dufour, F Moutou, The Brucellin skin test as a tool to discriminate false positive serological reactions in bovine brucellosis. Veterinary Research. ,vol. 28, pp. 365- 374 ,(1997)
J. McGiven, L. Hendry, D. Brown, J. Stack, L. Perrett, I. Mawhinney, The improved specificity of bovine brucellosis testing in Great Britain. Research in Veterinary Science. ,vol. 84, pp. 38- 40 ,(2008) , 10.1016/J.RVSC.2007.03.003
Klaus Nielsen, Diagnosis of brucellosis by serology. Veterinary Microbiology. ,vol. 90, pp. 447- 459 ,(2002) , 10.1016/S0378-1135(02)00229-8
R Pouillot, Ph Lescoat, B Garin-Bastuji, D Repiquet, P Terrier, G Gerbier, J.J Bénet, M Sanaa, Risk factors for false-positive serological reactions for bovine brucellosis in Saône-et-Loire (France) Preventive Veterinary Medicine. ,vol. 35, pp. 165- 179 ,(1998) , 10.1016/S0167-5877(98)00062-2
Jacques Godfroid, Annemarie Käsbohrer, Brucellosis in the European Union and Norway at the turn of the twenty-first century. Veterinary Microbiology. ,vol. 90, pp. 135- 145 ,(2002) , 10.1016/S0378-1135(02)00217-1
Jacques Godfroid, Claude Saegerman, Vincent Wellemans, Karl Walravens, Jean-Jacques Letesson, Anne Tibor, Alastair Mc Millan, Steve Spencer, Moëz Sanna, Douwe Bakker, Régis Pouillot, Bruno Garin-Bastuji, How to substantiate eradication of bovine brucellosis when aspecific serological reactions occur in the course of brucellosis testing. Veterinary Microbiology. ,vol. 90, pp. 461- 477 ,(2002) , 10.1016/S0378-1135(02)00230-4
C Saegerman, L De Waele, D Gilson, J Godfroid, P Thiange, P Michel, B Limbourg, T.K.-O Vo, J Limet, J.-J Letesson, D Berkvens, Evaluation of three serum i-ELISAs using monoclonal antibodies and protein G as peroxidase conjugate for the diagnosis of bovine brucellosis Veterinary Microbiology. ,vol. 100, pp. 91- 105 ,(2004) , 10.1016/J.VETMIC.2004.02.003
C. Saegerman, T.-K. O. Vo, L. De Waele, D. Gilson, A. Bastin, G. Dubray, P. Flanagan, J. N. Limet, J.-J. Letesson, J. Godfroid, Diagnosis of bovine brucellosis by skin test: conditions for the test and evaluation of its performance. Veterinary Record. ,vol. 145, pp. 214- 218 ,(1999) , 10.1136/VR.145.8.214