作者: Yoshihiro Ito , Ji Zheng , Yukio Imanishi
DOI: 10.1007/978-4-431-65883-2_69
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摘要: Insulin was immobilized on surface-hydrolyzed poly(methyl mcthacrylate) membrane anchorage-dependent and anchorage-indepedent cells were cultured the insulin-immobilized membrane. The growth of cell efficiently enhanced by very small amount insulin (1/10 to 1/100 free insulin) coimmobilization adhesion factors promoted effect. Although anchorage-independent not affected insulin, accelerated enhancing adhesion. In addition using a overexpressing receptor it revelaed that cellular receptors autophosphorylated after time lag needed for