Site specific immobilization of a potent antimicrobial peptide onto silicone catheters: evaluation against urinary tract infection pathogens

摘要: Bacterial colonization of urinary catheters is a common problem leading to Catheter Associated Urinary Tract Infections (CAUTIs) in patients, which result high treatment costs and associated complications. Due the advantages antimicrobial peptides (AMPs) compared most other molecules, an increasing number AMP-coated surfaces being developed but their efficacy hindered by suboptimal coating methods loss peptide activity upon surface tethering. This study aims address this issue employing methodic approach that combines simple selective chemical immobilization platform on silicone catheter with choice potent AMP, Lasioglossin-III (Lasio-III), allow site specific Lasio-III at effective concentration. The was chemically modified N-terminal cysteine residue facilitate cysteine-directed onto commercial via combination allyl glycidyl ether (AGE) brush polyethylene glycol (PEG) based coupling. amount immobilized determined be 6.59 ± 0.89 μg cm−2 Sulfo-SDTB assay. showed good against both Gram positive negative bacteria. properties were sustained for least 4 days post-incubation physiologically relevant environment artificial urine prevented biofilm growth E. coli faecalis. Adenosine tri-phosphate leakage propidium iodide fluorescence studies further confirmed membranolytic mode action peptide. To best our knowledge, first proof-of-concept reports AMP sulfhydryl coupling real surface.