The Nox1/Nox4 inhibitor attenuates acute lung injury induced by ischemia-reperfusion in mice.

摘要: Lung ischemia and reperfusion injury (LIRI) were mediated by several processes including over-production of reactive oxygen species (ROS) inflammatory activation. ROS generated nicotinamide adenine dinucletide phosphate (NADPH) oxidase (Nox) may play a pivotal role in pathophysiological changes range disease. However, it was poorly understood LIRI. Thus, the purpose our study to explore whether GKT137831, as special dual inhibitor Nox1 4, could alleviate LIRI mice model minimal dose. According protocol, this divided into two parts. The first part determine dose Nox1/4 attenuating via histopathology apoptosis analysis. Eighteen C57BL/6J male wild-type randomly sham, 2.5Nox+sham, 5.0Nox+sham, IR, 2.5Nox+IR 5.0Nox+IR groups. different group, pretreated with corresponding inhibitors or normal saline. After LIRI, results showed 5.0mg/kg be considered decreasing lung score number TUNEL-positive cells. second further verify benefit protective effects. Thirty-seven IR groups randomly. that expressions inflammatory, autophagy cytokines markedly elevated PH value declined after 5.0 mg/kg significantly attenuated cytokine production reflected immunohistochemistry, western blotting Q-PCR In conclusion, findings suggested contributed protect tissue damage suppression