The nuclear pore complex and lamina: three-dimensional structures and interactions determined by field emission in-lens scanning electron microscopy.

摘要: Abstract Three dimensional surface imaging was used to examine structural details of the nuclear pore complex. Subsurface structures were uncovered by detergent extraction, proteolysis, mechanical fracturing and combinations these. Features observed in this way mostly consistent with previous three-dimensional some novel observations. In addition cytoplasmic basket filaments attached each coaxial ring, we have radiating within central channel. New organisation are presented, showing that branch woven together form ring. The “central transporter” is shown a be regular, structure revealed after removal overlying internal filaments. lumenal ring visualised present evidence lamina may spoke Finally show there for structure, “star ring”, sandwiched between membrane.